To permanently disable video looper, i.e. to prevent it from ever starting on boot again, you can run a small script included with the video looper code. Connect to the Raspberry Pi in a terminal/SSH session and navigate to the folder where video_looper was downloaded, usualy the directory with this path /home/pi/pi_video_looper.
cd /home/pi/pi_video_looper
Now run the disable script by executing:
sudo ./disable.sh
Don’t worry if you see an error message displayed that video looper is already stopped (this might happen if video looper isn’t running when its disabled). After running the script above video looper will be disabled and should not run on boot.
With
sudo ./enable.sh
you can re-initiate the Video_Looper permanently again.
source:
https://learn.adafruit.com/raspberry-pi-video-looper?view=all